Low-cost high-throughput targeted sequencing for the accurate detection of respiratory tract pathogens

Low-cost high-throughput targeted sequencing for the accurate detection of respiratory tract pathogens

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Introduction: The footjoy weste herren current gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis by real-time reverse transcriptase polymerase chain reaction (RT-PCR) is limited by the number of genes that can be detected.In this study, we developed a low-cost and high-throughput next-generation sequencing technology that can overcome the limitations of real time RT-PCR.Methodology: A targeted sequencing panel (TSP) consisting of approximately 500 amplicons was designed.

This panel could simultaneously detect a broad range of gene loci of SARS-CoV-2, and genes for the most common infectious viruses that affect the respiratory tract, in a single run and could include up to 96 samples.Four hundred and forty-eight samples and 31 control samples were analyzed independently with both TSP and RT-PCR, and the results were compared for accuracy and other indicators.Results: TSP identified 50 SARS-CoV-2 positive samples with a 99.

33% match to RT-PCR results.It is not surprising that TSP also identified multiple infections from the 96 samples, whereas RT-PCR could not.Thus, TSP was able to accurately diagnose the samples which could not be identified based on single RT-PCR test.

Conclusions: Our data demonstrated that TSP is trailmaster challenger 200x a fast and accurate testing method for identifying multiple pathogen infections of the respiratory tract.